WL Gear Medium (+5% NaCl)
WL Differential Method is used for isolating bacterias encountered in brewing and industrial fermentation processes. Brief summary and Justification
WL (Wallerstein Laboratory) chemical media were developed by Green and Gray1, 2 in their study of varied fermentation techniques. An exhaustive study evaluating the methods of fermentation control procedures in worts, sodas, liquid yeasts and related fermentation items led to the development of these multimedia. At a pH of 5. 5, counts of viable bakers' yeast can be made within the WL Nutritional Medium. By adjusting the pH to six. 5, the medium is appropricate for obtaining matters of bakers' and distiller's yeast. The medium support the growth of bacteria, although unless the number of yeast cellular material is small the bacteria may not be recognized. Due to this limitation, Green and Gray developed WL Differential Method that prevents the growth of yeasts without inhibiting the expansion of bacterias present in drinks. The WL Differential agar agar plate is usually incubated anaerobically for regarding lactic chemical p bacteria and Pediococcus. Rules of the Procedure
Yeast extract is a source of trace elements, vitamins and amino acids. Peptone provides nitrogen, amino acids and carbon. Dextrose is the method to obtain carbohydrate. Monopotassium phosphate buffers the media. Potassium chloride, calcium chloride and ferric chloride are necessary ions that help to maintain osmotic balance. Magnesium sulfate and manganese sulfate are sources of divalent cations. Bromcresol green is a pH indicator. Agar agar is the strengthening agent in WL Chemical Medium and WL Differential Medium. Cycloheximide inhibits yeasts and conforms in WL Differential Medium. User Top quality Control
Difcoв„ў WL Chemical Medium or perhaps WL Differential Medium
Dried up Appearance: Light beige which has a greenish hue, freeflowing, homogeneous. Solution: almost 8. 0% option, soluble in purified normal water upon cooking. Solution is definitely blue to greenish blue, very a bit...
References: 1 ) Green and Gray. 1950. Wallerstein Lab. Petit. 12: 43.
2 . Green and Gray. 1950. Wallerstein Laboratory. Commun. 13: 357.
several. MacFaddin. 1985. Media for isolation-cultivation-identification-maintenance of medical bacteria,
vol. 1 ) Williams & Wilkins, Baltimore, Md.