п»їPLAN AND DESIGN LAB
Speculation: The crimson food colouring from 3 different brands of purple coloured candies do not contain the same dyes Purpose: To separate the dyes via three several brands of violet coloured goodies using conventional paper chromatography and compare the results received. Apparatus and Materials: Several identical cooking food tubes with stoppers and hooks, normal water, measuring cylinder, chromatography or newsprint, 3 different brands (A, W, C) of purple colored candies Technique: 1 . Cut three components of chromatography paper/ newsprint 2cm x 8cm 2 . Draw a range 1 . 5cm from one end of each document 3. Applying one document for each model of purple meals colouring, put ne spot of the same size in the middle of the line drawn.
4. Gauge the volume of normal water needed to reach about a 1 / 4 the height of the boiling pipe Pour this kind of Volume in to each conduit. Label the tubes A, B, C to match the brands. your five. Set up the investigations while shown in the diagram.
6th. When the water reaches the very best of the whitening strips, remove every single chromatogram and let it to dry 7. Note the shades and positions of the spots
Expected Effects: The crimson food coloring will individual into a number of colours each of which will form a spot for different positions in the chromatogram. If the shade of the places and their positions are the same for every single brand then this hypothesis applies and approved. Limitations/ Options for error: In the event newsprint is utilized instead of chromatography paper, the spots may well not separate completely. The areas of food colouring may well not have been precisely the same size. Assumptions: Water is definitely an efficient solvent for the several brands of violet food colouring.